| Matches by round |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 |
| Match list: | By date Latest | Player stats: |
| R | Date | Home | Score | Away | |
| 1 | 16/08/2009 | Tranmere Rovers | 5 - 0 | Scunthorpe Unit | |
| 2 | 23/08/2009 | Scunthorpe Unit | 1 - 3 | South Durham | |
| 4 | 06/09/2009 | Bradford City | 8 - 2 | Scunthorpe Unit | |
| 5 | 13/09/2009 | Scunthorpe Unit | 4 - 1 | Blackpool Wren | |
| 6 | 20/09/2009 | Darlington | 0 - 4 | Scunthorpe Unit | |
| 7 | 27/09/2009 | Scunthorpe Unit | 1 - 4 | Middlesbrough | |
| 8 | 04/10/2009 | Liverpool Feds | 5 - 1 | Scunthorpe Unit | |
| 9 | 11/10/2009 | Scunthorpe Unit | 0 - 6 | Salford | |
| 10 | 18/10/2009 | Wakefield | 1 - 1 | Scunthorpe Unit | |
| 11 | 25/10/2009 | Stockport Co | 1 - 0 | Scunthorpe Unit | |
| 13 | 22/11/2009 | Rochdale | 5 - 2 | Scunthorpe Unit | |
| 17 | 31/01/2010 | Scunthorpe Unit | 1 - 6 | Bradford City | |
| 19 | 14/02/2010 | Scunthorpe Unit | 2 - 0 | Tranmere Rovers | |
| 21 | 28/02/2010 | Blackpool Wren | 1 - 1 | Scunthorpe Unit | |
| 22 | 07/03/2010 | Scunthorpe Unit | 2 - 3 | Stockport Co | |
| 23 | 14/03/2010 | Middlesbrough | - | Scunthorpe Unit | |
| 24 | 21/03/2010 | Scunthorpe Unit | - | Rochdale | |
| 25 | 28/03/2010 | Salford | - | Scunthorpe Unit | |
| 26 | 04/04/2010 | South Durham | - | Scunthorpe Unit | |
| 27 | 11/04/2010 | Scunthorpe Unit | - | Liverpool Feds | |
| 28 | 18/04/2010 | Scunthorpe Unit | - | Darlington | |
| 30 | 02/05/2010 | Scunthorpe Unit | - | Wakefield |