| Matches by round |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 |
| Match list: | By date Latest | Player stats: |
| R | Date | Home | Score | Away | |
| 1 | 16/08/2009 | Darlington | 1 - 6 | Middlesbrough | |
| 2 | 23/08/2009 | Middlesbrough | 2 - 3 | Tranmere Rovers | |
| 4 | 06/09/2009 | Middlesbrough | 2 - 1 | Salford | |
| 5 | 13/09/2009 | Wakefield | 0 - 1 | Middlesbrough | |
| 7 | 27/09/2009 | Scunthorpe Unit | 1 - 4 | Middlesbrough | |
| 8 | 04/10/2009 | Middlesbrough | 0 - 1 | South Durham | |
| 9 | 11/10/2009 | Rochdale | 3 - 2 | Middlesbrough | |
| 12 | 01/11/2009 | Middlesbrough | 10 - 0 | Darlington | |
| 13 | 22/11/2009 | Middlesbrough | 3 - 1 | Liverpool Feds | |
| 14 | 06/12/2009 | Salford | 0 - 2 | Middlesbrough | |
| 16 | 24/01/2010 | Bradford City | 1 - 7 | Middlesbrough | |
| 18 | 07/02/2010 | Middlesbrough | 2 - 0 | Bradford City | |
| 22 | 07/03/2010 | Middlesbrough | 2 - 1 | Wakefield | |
| 23 | 14/03/2010 | Middlesbrough | 7 - 1 | Scunthorpe Unit | |
| 25 | 28/03/2010 | Middlesbrough | 0 - 2 | Rochdale | |
| 26 | 04/04/2010 | Middlesbrough | 1 - 1 | Stockport Co | |
| 27 | 11/04/2010 | Tranmere Rovers | 1 - 7 | Middlesbrough | |
| 30 | 02/05/2010 | Stockport Co | 0 - 2 | Middlesbrough | |
| 34 | 06/05/2010 | South Durham | 1 - 2 | Middlesbrough |