| Matches by round |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 |
| Match list: | By date Latest | Player stats: |
| R | Date | Home | Score | Away | |
| 1 | 16/08/2009 | Tranmere Rovers | 5 - 0 | Scunthorpe Unit | |
| 2 | 23/08/2009 | Middlesbrough | 2 - 3 | Tranmere Rovers | |
| 3 | 30/08/2009 | South Durham | 3 - 0 | Tranmere Rovers | |
| 4 | 06/09/2009 | Tranmere Rovers | 2 - 0 | Liverpool Feds | |
| 5 | 13/09/2009 | Rochdale | 5 - 1 | Tranmere Rovers | |
| 7 | 27/09/2009 | Bradford City | 7 - 3 | Tranmere Rovers | |
| 8 | 04/10/2009 | Tranmere Rovers | 2 - 0 | Wakefield | |
| 10 | 18/10/2009 | Tranmere Rovers | 3 - 3 | Stockport Co | |
| 11 | 25/10/2009 | Tranmere Rovers | 9 - 0 | Darlington | |
| 15 | 13/12/2009 | Tranmere Rovers | 0 - 2 | Rochdale |