| Matches by round |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 |
| Match list: | By date Latest | Player stats: |
| R | Date | Home | Score | Away | |
| 1 | 16/08/2009 | Darlington | 1 - 6 | Middlesbrough | |
| 2 | 23/08/2009 | Liverpool Feds | 8 - 0 | Darlington | |
| 3 | 30/08/2009 | Salford | 10 - 0 | Darlington | |
| 4 | 06/09/2009 | Darlington | 3 - 1 | Wakefield | |
| 5 | 13/09/2009 | Stockport Co | 3 - 1 | Darlington | |
| 6 | 20/09/2009 | Darlington | 0 - 4 | Scunthorpe Unit | |
| 7 | 27/09/2009 | South Durham | 3 - 0 | Darlington | |
| 8 | 04/10/2009 | Darlington | 2 - 7 | Rochdale | |
| 9 | 11/10/2009 | Bradford City | 11 - 0 | Darlington | |
| 11 | 25/10/2009 | Tranmere Rovers | 9 - 0 | Darlington | |
| 12 | 01/11/2009 | Middlesbrough | 10 - 0 | Darlington | |
| 18 | 07/02/2010 | Darlington | 2 - 4 | Blackpool Wren | |
| 23 | 14/03/2010 | Blackpool Wren | - | Darlington | |
| 24 | 21/03/2010 | Darlington | - | Bradford City | |
| 25 | 28/03/2010 | Darlington | - | South Durham | |
| 26 | 04/04/2010 | Darlington | - | Tranmere Rovers | |
| 27 | 11/04/2010 | Rochdale | - | Darlington | |
| 28 | 18/04/2010 | Scunthorpe Unit | - | Darlington | |
| 30 | 02/05/2010 | Darlington | - | Liverpool Feds |