| Matches by round |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 |
| Match list: | By date Latest | Player stats: |
| R | Date | Home | Score | Away | |
| 1 | 16/08/2009 | South Durham | 0 - 1 | Stockport Co | |
| 2 | 23/08/2009 | Scunthorpe Unit | 1 - 3 | South Durham | |
| 3 | 30/08/2009 | South Durham | 3 - 0 | Tranmere Rovers | |
| 4 | 06/09/2009 | Rochdale | 1 - 3 | South Durham | |
| 5 | 13/09/2009 | Bradford City | 0 - 1 | South Durham | |
| 7 | 27/09/2009 | South Durham | 3 - 0 | Darlington | |
| 8 | 04/10/2009 | Middlesbrough | 0 - 1 | South Durham | |
| 9 | 11/10/2009 | South Durham | 0 - 3 | Liverpool Feds | |
| 11 | 25/10/2009 | South Durham | 3 - 1 | Wakefield | |
| 12 | 01/11/2009 | Stockport Co | 0 - 0 | South Durham | |
| 15 | 13/12/2009 | South Durham | 2 - 1 | Bradford City | |
| 25 | 28/03/2010 | Salford | 0 - 3 | South Durham | |
| 26 | 04/04/2010 | South Durham | 4 - 1 | Scunthorpe Unit | |
| 27 | 11/04/2010 | Wakefield | 1 - 1 | South Durham | |
| 28 | 18/04/2010 | South Durham | 1 - 3 | Rochdale | |
| 35 | 22/04/2010 | Darlington | 0 - 11 | South Durham | |
| 29 | 25/04/2010 | South Durham | 1 - 1 | Salford | |
| 34 | 06/05/2010 | South Durham | 1 - 2 | Middlesbrough | |
| 31 | 09/05/2010 | Liverpool Feds | 2 - 2 | South Durham | |
| 32 | 16/05/2010 | Tranmere Rovers | 0 - 1 | South Durham |