| Matches by round |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 |
| Match list: | By date Latest | Player stats: |
| R | Date | Home | Score | Away | |
| 1 | 16/08/2009 | South Durham | 0 - 1 | Stockport Co | |
| 2 | 23/08/2009 | Scunthorpe Unit | 1 - 3 | South Durham | |
| 3 | 30/08/2009 | South Durham | 3 - 0 | Tranmere Rovers | |
| 4 | 06/09/2009 | Rochdale | 1 - 3 | South Durham | |
| 5 | 13/09/2009 | Bradford City | 0 - 1 | South Durham | |
| 7 | 27/09/2009 | South Durham | 3 - 0 | Darlington | |
| 8 | 04/10/2009 | Middlesbrough | 0 - 1 | South Durham | |
| 9 | 11/10/2009 | South Durham | 0 - 3 | Liverpool Feds | |
| 11 | 25/10/2009 | South Durham | 3 - 1 | Wakefield | |
| 12 | 01/11/2009 | Stockport Co | 0 - 0 | South Durham | |
| 15 | 13/12/2009 | South Durham | 2 - 1 | Bradford City | |
| 19 | 14/02/2010 | Blackpool Wren | 1 - 2 | South Durham | |
| 23 | 14/03/2010 | Salford | - | South Durham | |
| 24 | 21/03/2010 | Liverpool Feds | - | South Durham | |
| 25 | 28/03/2010 | Darlington | - | South Durham | |
| 26 | 04/04/2010 | South Durham | - | Scunthorpe Unit | |
| 27 | 11/04/2010 | Wakefield | - | South Durham | |
| 28 | 18/04/2010 | South Durham | - | Rochdale | |
| 29 | 25/04/2010 | South Durham | - | Salford | |
| 30 | 02/05/2010 | South Durham | - | Blackpool Wren |