| Matches by round |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 |
| Match list: | By date Latest | Player stats: |
| R | Date | Home | Score | Away | |
| 5 | 13/09/2009 | Bradford City | 0 - 1 | South Durham | |
| 5 | 13/09/2009 | Liverpool Feds | 1 - 3 | Salford | |
| 5 | 13/09/2009 | Rochdale | 5 - 1 | Tranmere Rovers | |
| 5 | 13/09/2009 | Scunthorpe Unit | 4 - 1 | Blackpool Wren | |
| 5 | 13/09/2009 | Stockport Co | 3 - 1 | Darlington | |
| 5 | 13/09/2009 | Wakefield | 0 - 1 | Middlesbrough |