| Matches by round |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 |
| Match list: | By date Latest | Player stats: |
| R | Date | Home | Score | Away | |
| 2 | 23/08/2009 | Liverpool Feds | 8 - 0 | Darlington | |
| 2 | 23/08/2009 | Middlesbrough | 2 - 3 | Tranmere Rovers | |
| 2 | 23/08/2009 | Rochdale | 4 - 2 | Stockport Co | |
| 2 | 23/08/2009 | Salford | 5 - 0 | Blackpool Wren | |
| 2 | 23/08/2009 | Scunthorpe Unit | 1 - 3 | South Durham | |
| 2 | 23/08/2009 | Wakefield | 2 - 3 | Bradford City |