| Matches by round |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 |
| Match list: | By date Latest | Player stats: |
| R | Date | Home | Score | Away | |
| 1 | 16/08/2009 | Blackpool Wren | 1 - 1 | Liverpool Feds | |
| 1 | 16/08/2009 | Darlington | 1 - 6 | Middlesbrough | |
| 1 | 16/08/2009 | Rochdale | 5 - 2 | Wakefield | |
| 1 | 16/08/2009 | Salford | 1 - 1 | Bradford City | |
| 1 | 16/08/2009 | South Durham | 0 - 1 | Stockport Co | |
| 1 | 16/08/2009 | Tranmere Rovers | 5 - 0 | Scunthorpe Unit |